Overexpression of BEH2 regulates a much broader range of gene expression than BZR1

Based on the distinct phenotypes of BEH2-overexpressing plants, we compared the functional roles of BZR1 and BEH2. The BEH2 is a stronger transcriptional regulator of BR-responsive gene expression than BZR1. It appears that BEH2 also regulates gene expression in a BR-independent manner. Overall design: To examine whether BZR1 and BEH2 have different transcriptional regulations in BR signaling, we performed RNA-Seq analysis in Col-0, Col-0 treated with BL, 35S-BZR1-YFP, and 35S-BEH2-YFP.