Single-nucleus transcriptomics of inducible Ngly1-/- cerebellum

To explore the cell type-specific effects of NGLY1 deficiency and the STING pathway in the cerebellum, we performed single-nucleus RNA sequencing (snRNA-seq) of cerebella from Ngly1fl/fl, iNgly1-/- and iNgly1-/-Sting1-/- mice Overall design: Eight-week-old Ngly1fl/fl, iNgly1-/- and iNgly1-/-Sting1-/- male mice (2 mice/genotype) were sacrificed and cerebella were collected and frozen in liquid nitrogen. Nuclei were isolated from frozen cerebella and droplet-based snRNA-sequencing was performed using 10x Genomics.