Transcriptome profiling of environmental relevant low concentrations (0.4 ng/L) of nano-Palladium in Danio rerio

The effects of nano-palladium (0.4 ng per L) in zebrafish, Danio rerio, was determined using transcriptome sequencing. The purity of mRNA from the control and nano-Pd exposed fishes were checked and the processed reads were mapped with the reference genome of Danio rerio. Around 64792 genes were expressed in Treatment II respectively with respect to the total reads. There were 3153 differentially expressed genes in Control vs Treatment II with 2900 up-regulated and 253 down-regulated genes. Also, for Treatment I vs Treatment II, 3347 genes were significantly differentially expressed with 2101 up-regulated and 1246 down-regulated genes. The up-regulation and down-regulation of the genes were evident that the nano-Pd can affect the physiological and endocrine functions of zebrafish.