BIO407 Group6 Widefield images of DFX treated cells

## Study: This data is part of the practical course BIO407 2025 at the University of Zurich, titled 'Advanced Microscopy: From preparation to data and visualization'. The effects of Deferasirox (DFX) on mitochondria morphology were examined using different microscopy techniques. Course: BIO407 group-number: -1: -Jessica Goetschi -Alexa Fry -Luca Vogt ## Study Component: Job: Widefield Microscopy / High Content Screening Task: 7 Using a widefield microscope, high-content imaging was performed on Deferasirox treated and untreated cells. ## Biosample: COS-7 cells ## Specimen: Four different conditions: -Control 1: No treatment -Control 2: 5h DMSO treatment -Condition 1: 3h 200uM DFX treatment -Condition 2: 5h 200uM DFX treatment Cells were fixed with 4% PFA, 0.05% GTA, 0.5% Saponin in PHEM. Cells were labeled with DAPI, TOMM20-Alexa488, Tubulin-Alexa568, SiR-Actin. ## Image Acquisition: Images were acquired using a LeicaTHUNDER Imager 3D Live Cell on the Leica DMi8 platform. Acquired channels: Channel 1: DAPI, 390nm excitation, 460/80nm emission Channel 2: Alexa488-TOMM20, 475nm excitation, 535/70nm emission Channel 3: Alexa568-Tubulin, 555nm excitation, 590/50nm emission Channel 4: SiR-actin, 635nm excitation, 642/80nm emission Objectives used: 10x LEICA HC PL FLUOTAR air objective, 0.32NA 20x LEICA HC PL FLUOTAR L air objective, 0.40NA 40x LEICA HC PL APO CORR air objective, 0.95NA 63x LEICA HC PL APO CS2 oil immersion objective, 1.40NA ## Image Data: Project Name Convention: [Blockcourse]_[Group]_[Microscope]_[Raw/Processed Image]_[DDMMYYYY]_[Sample]_[Task].lif Image Name Convention: [Magnification x Immersion]_[Additional Information]_[Task] Additional Information: - RFC: Focusing each channel individually during image acquisition - Sampling: oversample, nyquist, undersample - XXXms_exposuretime: time in ms for exposure time - automatic_acuiqistion: Automatic Acquistion for screening of different conditions - Position 1-9: 5h DMSO treatment - Position 10-18: 3h 200uM DFX treatment - Position 19-27: 5h 200uM DFX treatment - Position 28-36: No treatment BIO407_G6_LeicaThunder_Raw_04032025_S6.1_Task1.lif - 10xDry_task1.5 BIO407_G6_LeicaThunder_Raw_04032025_S6.1_Task2.lif - 10xDRY_RFC_task2.1 - 20xDRY_RFC_task2.2 - 63xOIL_task2.3 BIO407_G6_LeicaThunder_Raw_04032025_S6.1_Task3.lif - 63xOIL_oversample_task3.1 - 63xOIL_nyquist_task3.1 - 63xOIL_undersample_task3.2 BIO407_G6_LeicaThunder_Raw_04032025_S6.1_Task4.lif - 63xOIL_200ms_exposuretime_task4.1 - 63xOIL_400ms_exposuretime_task4.1 - 63xOIL_500ms_exposuretime_task4.1 BIO407_G6_LeicaThunder_Raw_04032025_S6.1_Task7.lif - 40xAIR_automatic_acquisition_task7_PositionX