Intestinal flora date-Group_NCD_HFD_LFBE.zip

All high-quality sequencing reads were clustered
using USEARCH (v7.0.1090) with 97% similarity, and operational taxonomic units (OTUs)
were obtained with a certain threshold. Each OTU was selected as the
representative sequence and subjected to RDP Classifier software (v2.2) for
taxonomical assignment. The number of sequences per sample was corrected for
differences in sequencing depth between samples by rarefication, that is, the
same number of reads is randomly subsampled in each sample. Second, the
absolute number of sequences of each OTU in each sample was converted to
relative abundance to reduce the effect of differences in sequence reads. The
representative sequences, together with the abundance data, were used for
taxonbased analysis.