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Sequence sets, alignments and raw tree files for the structure-guided evolutionary analysis of group I introns

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https://figshare.com/articles/dataset/Sequence_sets_alignments_and_raw_tree_files_for_the_structure-guided_evolutionary_analysis_of_group_I_introns/28227962
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Datasets, alignments and tree files used to create figure 3 and supplementary figure S1 of the paper: 1. Entire set of analyzed introns (GIISSD database + introns identified in Bastillevirinae). 2. Non-redundant introns (cd-hit -i GIISSD_and_Bastille.fasta -o GIISSD_and_Bastille.unique_099.fasta -c 0.99 -s 0.99 -M 1000). 3. Original structure-guided alignment of the non-redundant intron set and RFAM group I model (cmalign -o Unique_vs_RF00028.sto --dnaout --mxsize 5140 --cpu 30 --matchonly --outformat Stockholm RF00028.cm GIISSD_and_Bastille.unique_099.fasta) and a version converted to fasta (./sto2fasta.py -s Unique_vs_RF00028.sto). 4. Pruned alignment (sites with more than 50% gaps removed using Geneious "mask alignment" tool, poorly aligned introns filtered out by: ./prune_alignment.py -f Unique_vs_RF00028.fasta -l 50). 5. Maximum likelihood and consensus trees of conserved intron elements (iqtree -nt AUTO -s Unique_vs_RF00028.pruned.fasta -alrt 1000 -bb 1000). (Scripts used in the study are available from https://github.com/zwmuam/Bastilevirinae_introns)
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2025-01-17
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