In vivo imaging of cellular processes in the pancreas of live GFP–LC3 mice starved for 24 h (see also Movie S5).

(A) Pancreatic acinar cells (left) and vasculature (right). (B) Time-lapse images of autophagosome movements in the boxed region (yellow) in A at the indicated times. Two GFP–LC3 dots exhibited distinct behaviors; one showed rapid displacement (yellow arrow), while the other remained stationary (yellow arrowhead). (C) Time-lapse images of an event similar to exocytosis in the boxed region (magenta) in A at the indicated times. Magenta arrows designate the protrusion-like profile. (D) Time-lapse images of pancreatic microcirculation obtained from the boxed region (white) in A at the indicated times. Individual cells appeared as dark foci in the vasculature. White arrowheads mark progress of a single cell. Scale bars, 20 µm (A), 2 µm (B) and 5 µm (C, D).