Distance-dependence seed set of Vasconcellea chilensis

Plant reproductive failure is a critical concern for conserving rare and endangered species that typically have low-density and sparse populations. One important factor contributing to reproductive failure is the spatial arrangement of plants within a population, which can lead to isolation and negatively affect seed output, particularly in obligate outcrosses. Additionally, this effect can be compounded by plant size. Here, we investigate how plants' spatial distribution and size influence the reproductive success of Vasconcellea chilensis, a threatened papaya species from northern Chile. Using flower exclusion experiments, we first examined whether V. chilensis can produce seeds via apomixis. We then used Spatial Point Pattern Analysis (SPPA) in three populations to examine the spatial arrangement of plants in three populations, and, finally, we assessed whether plant size and mate distance influence the reproductive success of this plant species. V. chilensis is a dioecious shrub una..., Vasconcellea chilensis (Planch. Ex A. Dc.) Solms 1889 (Caricaceae). Dioecious shrub with pistillate flowers (female) and staminate flowers (male).

Population Surveyed: Conchillas (CCH), Conchalí (CNL), and Puntilla Las Vacas (PLV).

Apomixis: We excluded pistillate flowers with mesh bags and marked untreated flowers as controls. After five to six months, we collected fruits and counted their seeds. 

Seed set: We collected five fruits from all female plants located in each population. For each plant, we calculate the seed set by dividing the mean number of seeds (in five fruits) by the mean number of ovules (in five flowers). 

Spatial Analyses: Individual plants were geo-referenced in the three populations. In each population, we run Spatial Point Pattern Analyses (SPPA) using Programita software. First, we calculate a univariate pair-correlation function (g(r)) separately for each sex, and second, we calculate a bivariate pair-correlation function (g12(r)) among both sexes (see d..., , # Distance-dependence seed set of *Vasconcellea chilensis*

[https://doi.org/10.5061/dryad.ttdz08m4s](https://doi.org/10.5061/dryad.ttdz08m4s)

We present the results of apomixis experiments, Spatial Point Pattern Analyses (SPPA), isolation, and Seed set of *Vasconcellea chilensis.*

## Description of the data and file structure

This repository contains data and code for the following manuscript: García-Guzmán, P., Loayza, A.P., Carvajal, D.E., and G. Carozzi-Figueroa. Lonesome plants: How isolation affects seed set of a threatened dioecious shrub. *Ecology and Evolution*.

The following folders and files are included:

### 1. Isolation (folder):

* Iso_Conchali.txt / Iso_Conchillas.txt / Iso_PLasVacas.txt / Isolation_All.txt = Datasets contain information for each female plant of plant size (Volume in m^3); brood size (Bsize), Seed set (Sset), Nearest male neighbor distance (NND) and the sum of all distances to male plants (SMD).
* Isolation.R = Rcode for lineal model fitting
* ...