Figures A-C.

Figure A. Phosphorylation patterns in RPPA versus RIPA cell lysates. (A) T80 cells were treated with 3000 IU/ml IFN-2α from 15 minutes to 24 hours. Cells were harvested using RPPA or RIPA lysis buffer. Lysates were analyzed by western blotting with the indicated antibodies (n = 2). (B) T80 cells were transfected with empty pcDNA3 plasmid (“pcDNA3”) or transfection reagent only (“mock”). Cell lysates were harvested from 6 to 48 hours post-transfection with RPPA or RIPA lysis buffer and analyzed via western blotting with the indicated antibodies (n = 2). Figure B. Inhibition of MAP kinase activity does not alter PLSCR1 protein upon dsDNA transfection. T80 cells were pre-treated with 10 μM U0126 for at least 2 hours prior to and after 6 hours post-transfection with empty plasmid. Lysates were collected at 24 and 48 hours post-transfection and analyzed via western blotting with the indicated antibodies (n = 3). Three replicates are presented (1–3). Figure C. STAT3 knockdown does not alter PLSCR1 induction upon dsDNA transfection. T80 cells were transfected with siRNA targeting STAT3 followed by empty plasmid pcDNA3 transfection for 24 hours. Cell lysates were then analyzed by western blotting with the indicated antibodies (n = 4). Four replicates are presented (1–4).
(PPTX)