Alternative splicing of genes during neuronal differentiation of NT2 pluripotential human embryonal carcinoma cells. (CBX132). unidentified

Multiple transcripts can be generated from a single gene by alternative splicing (AS). AS particularly occurs in the mammalian nervous system and plays an important role during neuronal differentiation in generating biological and functional diversities. To understand functions of genes, it is necessary to analyze mRNA diversity. Using human NT2 teratocarcinoma cells which differentiates toward a neuron by all-trans retinoic acid (RA) induction, we analyzed the mRNA diversity. Because their genetic risk factor was same, we discovered mRNA diversity during neuronal differentiation depending on the environmental risk factor. First, using DNA microarray of NT2 cells after RA induction at four time points (0, 1, 2 and 7 days), 40, 109 and 356 probes from which the difference was detected at the expression level were extracted on each time point respectively. Next, we identified genome loci of extracted probes. Then, it has been understood that selected 438 probes covered 365 genes (genome loci) and we analyzed the mRNA diversity of them. As a result, 279 genes which correspond to about 76% of our selected genes produced multiple protein-coding transcripts by AS. By GO classification analysis, we found that one of characteristic function of our selected genes was ?gtranscription regulator activity?h. We analyzed the mRNA diversity of transcription factors changed expression level by RA induction and compared the expression profile of protein-coding mRNAs transcribed from these genes by AS using realtime PCR. So, we confirmed mechanisms that one gene transcribes suitable protein coding mRNAs by AS during neuronal differentiation responding to the environmental risk factor.