<b>Dataset of GPi Deep Brain Stimulation in a Hemi-Parkinsonian rat model</b>

Dataset of GPi Deep Brain Stimulation in a Hemi-Parkinsonian rat model Version 0.0 Intermittent stimulation influences efficacy of deep brain stimulation on motor symptoms in hemi-Parkinsonian rats Contributed by M. M. Ostad Ali Akbary, A. Dini, and A. Erfanian (2025) Summary In this study, we evaluated the effects of various stimulation parameters on motor symptoms in a hemi-parkinsonian rat model during GPi-DBS. We assessed motor performance across a range of DBS frequencies, amplitudes, and pulse-widths. We also introduced an intermittent stimulation paradigm to reduce the delivered charge compared with continuous stimulation meanwhile improving DBS efficacy. A. Animal The experiments were conducted on 16 Wistar rats (male, 300–400 g). The animals were kept in a controlled environment with a 12-hour light‒dark cycle and unrestricted access to food and water. Ethical approval for all animal-related procedures was obtained from the Animal Care and Ethics Committee of the Iran Neural Technology Research Center, which is affiliated with the Iran University of Science and Technology. The study adhered to the recommended guidelines and protocols for the appropriate care and utilization of laboratory animals. B. LesioningThe rats were initially anesthetized via the intraperitoneal injection of a ketamine‒xylazine mixture (100 mg/kg ketamine and 12 mg/kg xylazine). Animal breathing, reflexes, and the level of anesthesia were monitored throughout the duration of the surgery. The depth of anesthesia was assessed by a lack of response to the hind limb or tail pinch. If there was a reaction, then half of the dose was repeated. To generate PD models, a 10 µl solution of 6-hydroxydopamine (6-OHDA) (Sigma Aldrich, GmbH, USA) was prepared in saline at a concentration of 2 mg/ml in 0.01% ascorbic acid and injected into the medial forebrain bundle (MFB, coordinates from bregma: A 2.76 mm, L 1.9 mm, H 8.3 mm) of the rats via a 10 µl microsyringe (80300, Hamilton, United States) with a 26-gauge needle. Two weeks after surgery, the extent of Parkinson's disease in each rat was assessed by injecting a solution of 0.05 mg/kg apomorphine (Sigma Aldrich, GmbH, USA, diluted in 0.2% ascorbic acid). The rats that exhibited a minimum of three anti-clockwise turns per minute were selected as well-lesioned rats. C. Electrode ImplantationThe lesioned rats then underwent stereotactic surgery for electrode implantation while being maintained under a ketamine‒xylazine mixture (100 mg/kg ketamine and 12 mg/kg xylazine). Each rat was implanted with a custom-made, four-electrode stainless steel stimulating microelectrode array into the right entopeduncular nucleus (EP), which corresponds to the globus pallidus internus (GPi, coordinates from bregma: A 2.64 mm, L 2.9 mm, H 7.7 mm). The stimulating microelectrode array was constructed by twisting four wires (impedance of 10 kΩ, diameter of 152 µm, AM system, 793500). To select a pair of electrodes that produced the most effective behavioral response and the fewest side effects during bipolar stimulation, an initial test was performed for each lesion rat. Different pairs of electrode wires were tested during bipolar stimulation, and the effective pair was selected. D. Deep Brain Stimulation DataWe captured video recordings from each rat to analyze the effects of various deep brain stimulation (DBS) parameters, including frequency, amplitude, pulse-width, and intermittent stimulation. These videos were processed with DeepLabCut to track the positions of each rat’s head and back. MATLAB was then used to determine the number of rotations performed during each trial, providing a quantitative measure of the effects of different DBS parameters. Conditions for using the data If you publish any work using this dataset, please cite it in the following recommended format: M. M. Ostad Ali Akbary, A. Dini, and A. Erfanian, “Intermittent Stimulation influences the efficacy of Deep brain stimulation on Motor Symptoms in Hemi-Parkinsonian Rats”. Data files organization The main MATLAB data file (.mat) is organized into five main fields: Frequency, Amplitude, Pulse-Width, Intermittent Stimulation, and Threshold as follows: · Frequency: The Frequency field contains ten subfields, each corresponding to a different evaluated frequency value (with a constant amplitude [I = I0] and pulse-width [65 µs]). Within each frequency subfield, there are ten individual rat fields, and each rat field contains three trial files (trial 1, trial 2, and trial 3). Every .mat file stores the positional data of the rat’s head and back from a 30-second video recording. · Amplitude: The Amplitude field is organized into five subfields, each representing a distinct evaluated amplitude (with constant frequency [130 Hz] and pulse-width [65 µs]). Each amplitude subfield includes six rat fields, and within each of these, three trial files (trial 1, trial 2, and trial 3) are provided. Each .mat file corresponding to an experimental trial captures the position data of the rat’s head and back from a 30-second video recording. · Pulse-Width: Within the Pulse-Width field, there are five subfields corresponding to different evaluated pulse-width values (maintaining constant frequency [130 Hz] and amplitude [I = I0]). Each subfield is further divided into six rat fields, and each rat field holds three trial files (trial 1, trial 2, and trial 3). Each .mat file corresponding to an experimental trial captures the position data of the rat’s head and back from a 30-second video recording. · Intermittent Stimulation: The Intermittent Stimulation field is divided into two categories based on the on-time of stimulation. For the 2-second on-time condition, the field includes six subfields representing different evaluated off-time values (with constant frequency [130 Hz], amplitude [I = I0], and pulse-width [65 µs]). Inside each off-time field, there are eight individual rat fields, each containing three trial files (trial 1, trial 2, and trial 3) with position data from a 30-second video recording. For the 3-second on-time condition, the field comprises three subfields for different evaluated off-time values (under the same constant parameters), where each off-time field contains five rat fields. Each .mat file corresponding to an experimental trial captures the position data of the rat’s head and back from a 30-second video recording. · Threshold: The Threshold field consists of eight subfields, each corresponding to an individual rat. Within these fields, the number of trial files varies according to the number of experiments conducted to achieve the threshold (with constant frequency [130 Hz] and pulse-width [65 µs]). Each .mat file corresponding to an experimental trial captures the position data of the rat’s head and back from a 30-second video recording. Data format The dataset is provided in MATLAB format (.mat). Each .mat file contains a matrix of dimensions n × m, where: · n: Represents the number of frames in the 30-second video recording. · m: Represents the positional data for the rat’s head and back in each frame. Specifically, the matrix contains: (x, y) coordinates for the rat’s head,(x, y) coordinates for the rat’s back,A likelihood value for each head and back position. This value indicates how confident the algorithm is that the labeled point is the true landmark, with values closer to one being more confident.