Additional file 9 of Epigenetic reprogramming-induced guanidinoacetic acid synthesis promotes pancreatic cancer metastasis and transcription-activating histone modifications

Additional file 9: Fig. S1. A high level of intracellular GAA wasassociated with PDAC liver metastasis. Fig. S2. GAA promoted liver metastasis of PDAC. Fig. S3. Knockdown of GATM suppressed livermetastasis of PDAC. Fig. S4. Three-dimensional epigenomereprogramming upregulates GATM expression promoting PDAC metastasis. Fig. S5. Metabolic alteration after GAAmetabolism disturbance. Fig. S6. GAA metabolism promotes HMGA-induced EMTvia upregulated MYC expression. Fig. S7. GAA promotes H3K27ac modifications atcell cycle and apoptosis-related genes. Fig. S8. GAA promotes H3K4me3 ofmetastasis-related genes by upregulating histone methyltransferases expression. Fig. S9. GAA metabolism promotes H3K27acmodification at the enhancers of the MYC gene.