Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and OsWRKY65-overexpression transgenic Transcriptomes

The revolutionary next-generation sequencing (NGS) analysis has opened a new era of analyzing signaling pathways. This study aims to elucidate the correlation of genes involved with WRKY65. The transcriptome analysis was performed in 2-week-old OsWRKY65-overexpression plants and wild-type (WT) through transcriptome deep sequencing (RNAseq). Differentially expressed genes (DEGs) were analyzed by filtering through fold change (FC) greater than or equal to 2 and P-value less than 0.05. Applying these criteria, Over 1,000 DEGs were analyzed in the OsWRKY65-overexpression plant compared to WT. Interestingly, some DEGs were analyzed as phytohormone-related genes. This result indicated that the OsWRKY65 might have a role in the phytohormone signaling pathway. As the OsWRKY65 is one of the WRKY superfamily, we analyzed the FC of the WRKY family. Among the family, few genes showed DEGs, and these results indicated that OsWRKY65 might involve in the phytohormone pathway and related to WRKY transcription factor. Overall design: mRNA expression profiles of the WT and OsWRKY65-2 by Illumina NovaSeq 6000.