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Results in Ct of SYBR Green real-time PCR with Tm 44.6°C, 50.2°C and 55°C.

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All plasmids, at 10−5 dilution each, were tested with 2 µM of each primer. Cells filled in bold indicate lowest Ct value for each plasmid. ‘C’: number of plasmid copies used as template.

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2014-03-20
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