Gastruloids generated without exogenous Wnt activation develop anterior neural tissues [EmbryoidsTruseq2]

Here we describe an approach to generate gastruloids that have a more complete antero-posterior development. We used bioengineered hydrogel microwell arrays to promote the robust derivation of mouse ESCs into post-implantation epiblast-like (EPI) aggregates in a reproducible and scalable manner. These EPI aggregates break symmetry and axially elongate without external chemical stimulation. Inhibition of WNT signaling in early stages of development leads to the formation of gastruloids with anterior neural tissues. Testing the effect of XAV939 on the development of mouse epiblast-like aggregates.