Taiwania

Spectral indices to rapidly monitor chloroplast distribution patterns of three cecidomyiid galls The objective of this research was to study altered distributions of chloroplasts in cecidomyiid galls derived from the leaves of Machilus thunbergii Sieb. & Zucc. and Litsea acuminata (Blume) Kurata using confocal laser-scanning microscopy and transmittance spectra. Chloroplasts in all gall tissues gradually decreased in the direction from the exterior toward the inside larval chamber based on Integrated Optical Density (IOD) and absorptivity indices, and chloroplast distributions in various gall tissues were species-specific. Different species of herbivorous insect dramatically altered chloroplasts in their host leaves. The spectral index was significantly and positively correlated with the IOD, and therefore, the transmittance spectra could be utilized as a tool to easily and quickly study the ecophysiology of galls.

本研究旨在通过共聚焦激光扫描显微镜和透射光谱，探究来自厚叶木（Machilus thunbergii Sieb. & Zucc.）和钝叶桢楠（Litsea acuminata (Blume) Kurata）叶片的蚜瘿中叶绿体的分布模式变化。根据综合光学密度（IOD）和吸收率指数，所有瘿组织的叶绿体从外向内幼虫室的方向逐渐减少，不同瘿组织的叶绿体分布具有物种特异性。不同种类的食草昆虫对其宿主叶片中的叶绿体产生了显著的影响。光谱指数与IOD显著正相关，因此，透射光谱可作为一种便捷快速研究瘿类生态生理的工具。