ITS1 amplicon sequencing of apple root DNA extracts for metabarcoding of the mycobiome
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https://www.ncbi.nlm.nih.gov/sra/ERP186268
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Within the scope of the ORDIAmur project the central experiment 12 included testing of 150 soils for apple replant disease severity by a biotest. DNA extracts of the apple plants used in the biotest were used as template for ITS1 short read amplicon sequencing to identify the fungal and fungal-like endophytes. Primers used: ITS1catta (ACCWGCGGARGGATCATTA) + ITS2ngs (TTYRCKRCGTTCTTCATCG) from Tedersoo and Anslan 2019 and Tedersoo et al. 2018
创建时间：
2025-12-10
