An animal study for evaluating the dose-dependent effects of fermented mixed grain enzyme food for adiposity and its metabolic disorders in high-fat-diet induced obese mice

From a long time ago, supplementation of fermented enzyme foods could have worked health effects on the body in the east nevertheless, only a few studies reported functions of them such as weight loss and metabolic syndrome. Thus, it is necessary to be verified whether supplementation of fermented enzyme foods can act in the body as a functional material. Therefore, we investigated the anti-obesity effects of fermented mixed grain with digestive enzymes (FMG) in high-fat diet induced mice. Sixty C57BL/6J mice were divided into six dietary groups and fed a normal diet (ND), a high-fat diet (HFD), Bacilus Coagulans group, steamed grain group, low-dose fermented mixed grain group(L-FMG), high-dose fermented mixed grain group (H-FMG) supplement for 12 weeks. After sacrificing, body weight and body fat mass in H-FMG group were significantly decreased compared to HFD group with a simultaneous decrease in plasma lipids. Also, H-FMG significantly decreased the blood glucose and improved the glucose tolerance compared to HFD group. Moreover high-dose FMG supplementation dramatically decreased the levels of inflammatory cytokines which secreted from adipocyte. Taken together, our findings suggest that H-FMG ameliorate high fat-diet induced obesity and its complication and could be used as a potential preventive agent for obesity. Four-week-old male C57BL/6J mice were obtained from Jackson Laboratory (Bar Harbor, ME, USA). All mice were individually housed under constant temperature (24 ◦C) and 12-h light/dark cycle. After a 1-week acclimation on a normal chow diet, the mice were then randomly divided into six groups, and each group was fed one of the following diets for 12 weeks: Normal diet (ND, 10% kcal from fat, n = 10); High fat diet (HFD, 40% of kcal from fat, n = 10); Bacilus Coagulans group (BC, HFD with 0.3% Bacilus Coagulans (w/w), n = 10); 0.3% Steamed grain group (SMG, HFD with 0.3% steamed grain (w/w), n = 10); Low-dose fermented mixed grain group (L-FMG, HFD with 0.3% fermented mixed grain (w/w)); High-dose fermented mixed grain group (H-FMG, HFD with 0.9 % fermented mixed grain (w/w)).