Longitudinal single cell transcriptional and epigenetic mapping of effector, memory, and exhausted CD8 T cells reveals shared biological circuits across distinct cell fates [scRNA-Seq]

Longitudinal scRNA-seq from LCMV-specific P14 CD8 T cells in LCMV-Armstrong and LCMV-Clone 13 infection LCMV-specific P14 cells were sorted from mice infected with LCMV-Arm or LCMV-Cl13 on days 8, 15, or 30 post infection. An additional group of mice was treated with anti-PDL1 every three days starting at day 16; cells were sorted at day 30. scRNA-seq libraries were prepared using 10x Genomics kits.