A comparison of workflows and consumable costs for various viral sequencing approaches.
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https://figshare.com/articles/dataset/_A_comparison_of_workflows_and_consumable_costs_for_various_viral_sequencing_approaches_/715721
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The ‘Custom’ protocol refers to the modified RNA-Seq method used in this study to create larger insert fragments. Alternative methods include Amplicon-seq and hybridisation capture (SureSelect Target Enrichment for Illumina Paired-End mRNA-Seq Library Prep; version 1.1). Failure rates are determined by failure to sequence at least one amplicon (<86% of the genome). The failure rate for SureSelect is not given as it was not performed in our study. Consumable costs are list price per sample and exclude sequencing.
*failure rate based on that observed with Sanger Sequencing.
**estimated cost for probes only. Extra cost incurred for Agilent library preparation kit plus additional reagents recommended by Agilent.
***linear amplification during SPIA reverse transcription has not been accounted for.
创建时间：
2013-06-10
