Frequency of T cell subsets.

Frequency of cytokine producing Ag85B and TB10.4 specific CD4 and CD8 T cells two weeks before and after M.tb challenge, as well as the frequencies of total CD4 and CD8 T cells, in rH4/Ad-H4 vaccinated mice.*The frequencies were obtained by FACS analysis on in vitro antigen stimulated splenocyte cultures from vaccinated mice (two weeks before challenge).**The frequencies were obtained by FACS analysis on in vitro antigen stimulated lung lymphocyte cultures from vaccinated and M.tb challenged mice two weeks after challenge.***Background staining from cells stimulated with medium alone has been subtracted.