Transcriptome analysis and H3K27ac Chip-seq analysis of Xenopus laevis regenerating nephric tubule

During tissue regeneration, cells first detect damage and then change the transcriptional status in their nuclei to reconstruct the tissue. Despite numerous developmental genes becoming reactivated during nephric tubule regeneration, cis-regulatory mechanisms for tissue regeneration are still unclear. We used Xenopus laevis nephric tubule regeneration as a model system. To chart the transcriptional landscape of the regenerating nephric tubule, we collected uninjured (day 0), regenerating (day 2), and regenerated proximal and intermediate tubules (day 5). Then, we performed the transcriptome analysis and H3K27ac Chip-seq analysis.