the effect of UIFSP on gene expression of WS1 cells after UVB exposure. the effect of UIFSP on gene expression of WS1 cells after UVB exposure

to investigate the function mechanism of UIFSP pretreatment in WS1 cells exposure to UVB,and how UIFSP regulate cell gene expression and signal transduction. Overall design: Comparative gene expression profiling analysis of RNA-seq data was used,which WS1 cells were divided into four groups, cell treated without UVB, cell exposed to UVB without UIFSP treatment, cell exposed to UVB with TAT-UIFSP 5 treatment,cell exposed to UVB with TAT-UIFSP 6 treatment.(Human skin fibroblast,WS1 cells grown in Dulbecco’s Modified Eagle Medium (DMEM, BI, Israel) supplemented with 1% antibioticsand 10% fetal bovine serum (FBS, BI, Israel) at 37 °C in a humidified incubator with 5% CO2. Before the UVB irradiation, cells were pre-treated with and without TAT-conjugated UIFSPs for 48 h. Cells were exposed to designated intensities of UVB (30 mJ/cm2) under a thin layer of PBS.)