in vitro fecal fermentation of sugar beet oligosaccharides

Goal was to examine how oligosaccharides generated from parent polysaccharides provide insight into both oligo- and polysaccharide degradation by gut microbiota. We conducted in vitro human fecal fermentations containing sugar beet oligosaccharides or sugar beet pulp. Samples were taken at 0, 10, 24 and 48 hours of fermentation, and changes of bacterial community composition were followed by 16S rRNA gene amplicon sequencing.