Additional file 9 of Detection and discovery of plant viruses in soybean by metagenomic sequencing

Additional file 9 Figure S9. Tobacco ringspot virus (TRSV) sequences confirmed by Sanger sequencing in green house grown N. benthamiana, and soybean infected plants following mechanical inoculation. Reverse transcription-polymerase chain reaction (RT-PCR) was performed in all 6 samples including mock-inoculated controls. The PCR products were visualized in a 1% agarose gel electrophoresis containing SYBR Safe DNA gel stain. The PCR products were cleaned using ExoSAP-IT PCR product cleanup reagent and sequenced on Sanger sequencing platform. Primer sequences were designed using Primer3 plus from assembled contigs and subsequently used in this study