George E. Palade (2011) CIL:7593, Rattus, hepatocyte. CIL. Dataset

This historic micrograph features the Golgi complex from a hepatocyte of a rat treated with ethanol to stimulate lipoprotein synthesis. The cisternae are distended and loaded with very low density lipoproteins (VLDL). VLDLs are also present in the smooth endoplasmic reticulum (SER). Mitochondria (Mt) also are visible in the field. This image is part of a set of two; the images are identical, but in this one, organelles do not have labels on them. It is also part of a set of four images focused on VLDL synthesis and secretion.

This type of image stimulated the development of a method (Ehrenreich JH, Bergeron JJM, Siekevitz P, Palade GE. 1973. Golgi fractions prepared from rat liver homogenate. I. Isolation procedure and morphological characteristics. J Cell Biol. 1973;59:45-72 and a companion paper, J Cell Biol,1973;59:73-88) for isolation of Golgi fractions that took advantage of the presence of newly synthesized lipoproteins moving through the Golgi to modify the density (lighten) Golgi fractions obtained after homogenization of the cell. This method results in more enriched Golgi fractions than could be obtained without the decrease in density provided by the lipoproteins.

Rats were starved overnight and then given 0.6 g ethanol per 100 g body weight in a 50% solution by stomach tube to stimulate lipoprotein synthesis and sacrificed 90 minutes later. Small blocks of liver tissue were fixed in 4% osmium tetroxide in 0.1 M cacodylate buffer pH 7.4 for 2 hours. The tissue was dehydrated and embedded in Epon. Sections were stained for 1 minute with ethanolic uranyl acetate and then alkaline lead citrate for 5 minutes.

This micrograph presented here originally appeared as Fig 4 in Bergeron JJ, et al., J Cell Biol. 1973;59:73-88.

The original image was created on September 14, 1972. The original 3.25 x 4 inch lantern slide was provided by George E. Palade; original is in the Palade Collection, University of California, San Diego. Digitization process: Lantern slide scanned at 1200 dpi in TIFF format, labeled in Photoshop then reduced to 600 dpi TIFF file (3500 x 2687 pixels) prior to conversion to JPEG2000 format.

此历史性的显微照片展示了经乙醇处理的鼠肝细胞中的高尔基体结构，以刺激脂蛋白合成。囊泡膨胀并充满极低密度脂蛋白（VLDL）。VLDL亦存在于平滑内质网（SER）中。线粒体（Mt）亦在该视野中清晰可见。该图像是两组图像中的一组，两组图像内容完全一致，但在此图像中，细胞器未标注标签。此外，该图像也是一组聚焦于VLDL合成与分泌的四幅图像之一。

此类图像激发了Ehrenreich JH、Bergeron JJM、Siekevitz P和Palade GE于1973年发表的方法（J Cell Biol. 1973;59:45-72及配套论文J Cell Biol, 1973;59:73-88）的发展，该方法利用新合成的脂蛋白通过高尔基体移动以改变（减轻）细胞匀浆后获得的高尔基体分组的密度。此方法产生的高尔基体分组比未使用脂蛋白减轻密度的情况下更为丰富。

实验鼠在饥饿过夜后，通过胃管给予每100克体重0.6克的50%乙醇溶液以刺激脂蛋白合成，并在90分钟后处死。小块肝组织在4%四氧化锇溶液和0.1 M草酸铵缓冲液（pH 7.4）中固定2小时。组织脱水和包埋于环氧树脂中。切片用乙醇铀酸乙酯染色1分钟，然后用碱性铅柠酸盐染色5分钟。

此处展示的显微照片最初作为Bergeron JJ等人（J Cell Biol. 1973;59:73-88）图4出现。

原始图像于1972年9月14日创建。原始的3.25 x 4英寸幻灯片由George E. Palade提供；原始图像存于加州大学圣地亚哥分校的Palade藏品中。数字化过程：幻灯片以1200 dpi分辨率扫描为TIFF格式，然后在Photoshop中进行标注，之后降低至600 dpi的TIFF文件（3500 x 2687像素），最后转换为JPEG2000格式。