Targeted RNA-sequencing of formalin-fixed paraffin-embedded breast cancer tissue samples

The presence of over-expressed receptors in triple-negative breast cancer (TNBC) leads to the absence of targeted therapy. Tumor budding (TB) has emerged as a recent prognostic indicator. It is essential to pinpoint genes unique to high TB cases to prevent tumor advancement and spread. In this study, we utilized TempO-Seq to profile transcriptomic data from breast cancer samples to identify biomarkers for high TB cases. The gene expression profile was subgrouped into 15 breast cancer (BCA) and 13 TNBC cases. BCA samples include 12 high and 3 low TB samples, while TNBC comprises 13 samples, with 11 high and 2 low TB patients.