Transcriptomic analysis revealed genes involved in response to cold stress in Prunus sibirica

Through transcriptome sequencing analysis of clones with strong freezing resistance (338) and weak freezing resistance (28) of Prunus sibirica,a total of 6001 significantly differentially expressed genes were obtained. Among them, there are 2070 up-regulated genes and 3,931 down-regulated genes. Differential genes are compared and screened, and WRKY, NAC, bHLH, MADS, MYB, Homeobox, F-box, ZEP, SBT, ERF, CAMTA, MDH, etc. Freeze-related gene family. Overall design: Using Prunus sibirica flower organs with strong frost resistance clone No. 338(treatment group) and weak frost resistance clone No. 28(control group) as the test materials, flowers were collected when the flower organs were subjected to frost (-4 °C). The clones were repeated 3 times, placed in liquid nitrogen, stored in an ultra-low temperature refrigerator (-80 °C), and transcriptome sequencing was completed by Nuovo Zhiyuan (Beijing) Co., Ltd.