Sanger sequence chromatograms of 17KDa and gltA Rickettsia genes demonstrating double peaks

A multilocus sequencing study of Torix group Rickettsia in arthropods providing evidence of co-infection with <i>Rickettsia</i>. During Sanger chromatogram analysis, double peaks were occasionally found at third codon sites from protein coding genes. This pattern was observed in 6/10 <i>Philotarsus californicus </i>individuals and in one member of each of the Psilidae, Sciaridae, Chironomidae and Diapriidae. Where double peaks were observed, this was found consistently across markers within an individual specimen. This pattern indicates co-infecting<i> Rickettsia</i> strains in hosts is a widespread phenomenon of the Torix group.