Analysis of 300 kb sequences from the centers of signal-rich and signal-poor human genomic DNA regions for the absence of Ensembl-annotated transcripts and ESTs for different GC isochore family combinations.

Chromosomes X and Y were excluded from the analysis. The criteria for signal-rich and signal-poor regions are described in the text. The P-values were calculated using the cumulative binomial distribution assuming the null hypothesis that there is no difference in the fractions of sequences that lack transcripts and ESTs between the signal-rich and signal-poor datasets. Isochore families: L1, 53 %, and the fraction of the genome for each isochore family were as described by Costantini et al. [28].