Inhibition of a pathologic RBM39/MLL1 epigenomic regulatory complex with a dominant-negative peptides disrupts cancer cell transcription, proliferation and survival

We report high-throughput profiling of RBM39 and MLL1 chromatin occupancy; H3K4me3 marks in T47D Control, T47D RBM39 KD and PME cells. By obtaining over four billion bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of RBM39, MLL1 and H3K4me3 in T47D and PME cells. We found that more than 4500 regions were cofound by RBM39 and MLL1. Examination of RBM39 and MLL1 chromatin occupancy in T47D cells.