Gene expression profile of in-vitro differentiated Dendritic Cells (DCs) from induced pluripotent stem cell (iPSC) and cord blood sources

Dendritic cells (DCs) are rare innate immune cells that are essential regulators of anti-tumour, anti-viral and vaccine responses by the adaptive immune system. We established a protocol to differentiate human dendritic cells from iPSCs and compare their behaviour with cord blood_derived equivalents. We used bulk RNA-seq analysis to characterise the different subsets of in vitro-generated DCs including DC1, DC2A and DC2B. In vitro-differentiated DC subsets from iPSC and cord blood CD34+ progenitors were isolated by Fluorescence-activated cell sorting (FACS) as Live HLADR+ CD11c+ CD141+ CLEC9A+ DC1, HLADR+ CD11c+ CD1c+ CD14- DC2A and HLADR+ CD11c+ CD1c+ CD14+ DC2B subsets and were analysed by bulk RNA sequencing.