Whole genome profiling of murine lymphocytes and statistical validation of a panel of immune molecules to predict vaccine efficacy against Mycobacterium tuberculosis

Transcriptional profiling of splenic lymphocytes derived from vaccinated mice, and ex-vivo exposed to M.tb.-infected macrophages in culture. Splenocytes from mice innoculated with Mycobacterium bovis BCG Pasteur (PAS), or M. bovis BCG Copenhagen (SSI), or heat killed BCG SSI (HK), or uninfected control, were ex vivo co-cultured with mouse bone marrow macrophages previously infected with M. tb. Mouse splenocytes from 3 different immunizations (plus Naive control), after ex-vivo exposure to MtB infected macrophages in co-culture, were re-isolated and RNA extracted for Illumina microarray