Effect of blood product - derived Cell Free Nucleic Acids (CFNA) on peripheral mononuclear cells gene regulation

PBMC were exposed to Cell Free Nucleic Acids (CFNA) extracted from blood products eligible for transfusion: Red Blood Cell units (RBC), Fresh Frozen Plasma units (FFP). PBMC alone exposed to the same extract from PBS were used as a control (PBMC+control). PBMC alone were also used as control. The used positive control was PBMC exposed to Lipopolysaccharide (LPS) exposition of PBMC to CFNA, RNA extarction and gene expression array (24h) PFC = FFP = Fresh Frozen Plasma units CE = RBC = Red Blood Cell units CP = PC = Platelet Concentrates