Establishment of bovine trophoblast stem cells [WGBS]

Here we report that a chemical cocktail (LCDM: hLIF, CHIR99021, DiM and MiH) previously reported for extended potential pluripotent stem cells enables the de novo derivation and long-term culture of bovine trophoblast stem cells (TSCs). Bovine TSCs exhibit transcriptomic and epigenetic features characteristic of trophectoderm cells from bovine embryos and retain developmental potency to differentiate into functional trophoblasts in vitro and in vivo Overall design: Whole genome bisulfite sequencing analysis was used to determined genome-scale DNA methylation patterns of bTSC, D7_TE, and D14_TE.