Whole Transcriptome analysis for porcine alveolar macrophage infected by African Swine Fever Virus

African swine fever virus (ASFV) is one of the most devastating swine pathogens characterized by nearly 100% mortality in naive herds and was recently emerged the in China. In this study, we generated the expression profile of porcine alveolar macrophages (PAMs) infected with a high pathogenic ASFV (Pig/Heilongjiang/2018 (Pig/HLJ/18) ASFV). Our data indicated that ASFV infection lead to a strong inhibition of host immunity but promote chemokine-mediated signaling pathway and neutrophil chemotaxis. Moreover, ASFV infection can modulate the host miRNA involved regulation network, leading to a significant increase of host metabolism related genes and acceleration of virus replication. Furthermore, ASFV-derived viral small RNAs (vsRNAs) can target some host immune response related genes. In conclusion, our transcriptome-wide data provide some insights into the regulatory mechanism during ASFV infection. Overall design: MicroRNA and mRNA profiles of porcine alveolar macrophages (PAMs) at different time-points post ASFV infection. MicroRNA and mRNA datasets were generated by deep sequencing, in triplicate, using Illumina Nextseq CN500 and Hiseq Xten platforms, respectively.