High-throughput time-resolved room-temperature serial crystallography on biomedically relevant proteins - towards molecular movies for biome
收藏ESRF Portal2028-01-01 更新2026-04-23 收录
下载链接：
https://doi.esrf.fr/10.15151/ESRF-ES-2010925158
下载链接
链接失效反馈官方服务：
资源简介：
We aim to perform high-throughput (HT) dynamic structural studies on biomedically relevant proteins enabled by our technology development for time-resolved serial microcrystallography (TR-SMX) at the ESRF ID-29 microfocus beamline. Implementation of novel polymer-based sample supports (“chips”) will provide efficient sample introduction for TR-SMX measurements, serving two scientific thrusts: (1) determination of dynamic structure-function relationships in bacterial virulence factors important for intercellular pathogen infection and survival; (2) elucidation of energy-transfer structural mechanisms in a novel fluorescent protein used in sensing intercellular phenomena. Our microfluidic chips for TR-SMX will enable rapid sequential data collection from hydrated microcrystals at room temperature under optimized, protein-specific conditions. Our HT approach is expected to produce protein “molecular movies”, facilitating mechanism-based therapeutics and vaccine development.
提供机构：
University of California, Davis, Chemical Engineering, 3112 Ghausi Hall, Ca 95616 Davis, Usa; Lawrence Livermore National Laboratory, Physical and Life Sciences Directorate, 7000 East Avenue, Ca94550 Livermore, Usa; Arizona State University, Center for Applied Structural Discovery, PO BOX 877401, 85287-740 Tempe, Usa; University of California at Davis, Dept Chemical Engineering & Material Science, 1 Shields Avenue, Ca, 95616 Davis, Usa
创建时间：
2028-01-01
