Gene expression data from the syngeneic mouse endometriosis model

Retrograde menstruation is considered a major reason for the development of endometriosis. The syngeneic transplantation mouse model is an endometriosis animal model that is considered to mimic retrograde menstruation. However, it remains poorly understood which genetic signatures of endometriosis are reflected in this model. Here, we employed an in vivo syngeneic mouse endometriosis model and identified differentially expressed genes (DEGs) between the ectopic and eutopic tissues using microarray analysis. Total RNA was isolated from the mouse ectopic cystic tissue or uterus using an RNeasy Lipid Tissue Mini Kit (Qiagen Inc., Hilden, Germany) (n = 5 animals per group). Purified RNA was labeled by using the GeneChip WT Plus Reagent Kit (Thermo Fisher Scientific, Inc.), then hybridized to a Clariom S Mouse Array (Thermo Fisher Scientific, Inc.).