Figure 5

Morphological analysis of human iPSC-derived activated astrocytes following 5 days TNF-α stimulation.(a) - Photomicrographs of human iPSC-derived astrocytes immunostained for vimentin (red), GFAP (green) and DAPI (blue); (b) – Quantification of vimentin immunostaining; (c) – Quantification of cell area in vimentin-stained cells. (d) – Percentage of astrocyte polarization, which cells were classified according to increase of length/width ratio of vimentin-stained labeling. (e) - Quantification of GFAP immunostaining expressed in arbitrary units of immunofluorescence (A.U); (f) - Quantification of DAPI-stained nuclei areas expressed as percentage of micrometers. Data are presented as means ± SEM from 4 cell lines in experiments performed in triplicates. ***P < 0.001. Unpaired Student´s t-test. Photomicrographs magnification: 200x. Calibration bar: 100 μm.