Human plasma peptidomics - Identification and quantification of plasma free salusin-β, an endogenous parasympathomimetic peptide

We have improved our ultrahigh-yield method, which we named “differential solubilization" (J Proteome Res 9, 2010, 1694-1705), to extract and analyse low-molecular-weight plasma peptides, and initiated a large-scale sequencing of circulating native peptides at concentrations as low as the picomolar level using less than 200 μl of human plasma.