A novel association of EZH2 and E2F1 promotes breast cancer cell proliferation through chromatin remodeling [ATAC-Seq]

This study provides transcription, histone modifications, chromatin accessibility in MDA-MB-231. We identified the non-canonical role of EZH2 by analyzing mutli-omics data. Overall design: ATAC-Seq were performed to survey chromatin accessibility in MDA-MB-231 with EZH2 or E2F1 presences. For knockdown experiment, cells at approximately 70-80% confluency were transduced with the lentivirus for 24 h and selected with puromycin (2 µg/ml) for 72 h.