Splenic T cell responses and DC maturation during WNV NY99 or H8912 infection.

A–B. Splenocytes of WNV NY99 or H8912-infected mice at day 8 and day 35 post-primary infection were cultured ex vivo with WNV peptides for 5 h (A) or with PMA plus ionomycin (B) for 24 h. A. Cells were harvested and stained for IFN-γ, and CD4 or CD8. B. Cell culture supernatant was collected and IL-10 was measured by Bioplex. C–D. DC maturation in WNV-infected BM-DCs. The percentages of CD80+ (C) and CD86+ (D) on BM-DCs were shown. Data are presented as means ± SEM, n = 3 or 4. **P<0.01 for WNV NY99 vs. WNV H8912.