BAP1 loss triggers DNA methylomic repatterning in highly aggressive Class 2 uveal melanomas [RNA-Seq]

The strong association between BAP1 mutations and highly aggressive Class 2 uveal melanoma (UM) suggests that epigenetic alterations may play a significant role in tumor progression. Thus, we characterized the impact of BAP1 loss on the DNA methylome in UM. Global DNA methylation was analyzed in 8 Class 1 and 4 Class 2 primary UMs and in 2 different UM cells (92.1, Mel202) engineered to inducibly deplete BAP1 with dox treatment. For the engineered cell lines, both uninduced and induced cell were analyzed with two replicates of all conditions.