MIC of anti-rpoD peptide-PNAs for quality control and clinical strains of Staphyloccus aureus in M-H broth culture.

arpoD, RNA polymerase sigma 70; PNA, peptide nucleic acid; PPNA means peptide conjugated PNA; The PNAs are written from their N to their C terminus, and the N terminus corresponds to the 5′ end of a conventional oligonucleotide; “K” indicates lysine, F indicates phenylalanine, “X” indicates 6-aminohexanoic acid, “B” indicates β-alanine, and eg1 indicates glycine; Scr means PNA with a scrambled base sequence (as control);bMinimal inhibitory concentrations (MIC) were the lowest PNA concentrations that prevented bacterial growth by visual inspection after overnight (24 h) growth from an inoculum of 105 CFU/mL. “VISA” is abbreviation for vancomycin-intermediate resistance Staphyloccus aureus; “MRSA” is abbreviation for methicillin-resistant Staphyloccus aureus; XIJING means clinical MRSA isolate from patients in Fourth Military Medical University affiliated XIJING hospital.