RNA-sequencing analyses of cancer cell lines from KPPC and PPSSC pancreatic tumors

RNA-sequencing analyses were performed on cancer cell lines from KPPC and PPSSC pancreatic tumors, so as to identify the changes of tumor immune microenvironment upon Trp53 and Smad4 deletion without KRAS mutation. We generated a novel transgenic mouse model (Trp53loxP/loxP;Smad4loxP/loxP;Pdx1-Cre) that harbors wild-type KRAS and loss of Trp53/Smad4 in the context of spontaneous PDAC. Primary cancer cell lines established from pancreatic tumors were analyzed by RNA-seq.