Characterization of WHc-CS VLPs carrying repeat and non-repeat B cell epitopes.

The listed WHcAg hybrid VLPs and full length rCS protein were used to immunize mice (2 doses; 20 μg and 10 μg in IFAd). Secondary antisera were pooled, serially diluted and analyzed by ELISA for binding to: solid phase WHcAg; repeat peptides (NANP)5 and (DPNANPNV)2; non-repeat peptides N1, Krich, CSP298-315; and rCS. Endpoint dilution titers are shown. Antisera were also evaluated by IFA on dry or viable sporozoites. The protective efficiency after in vivo challenge with 10,000 Pb/Pf sporozoites of mice immunized with the listed WHc-CS VLPs is also shown.Characterization of WHc-CS VLPs carrying repeat and non-repeat B cell epitopes.