CRISPR screen for interferon-alpha inducible inhibitors of yellow fever virus replication

Host cells produce interferon (IFN) in response to viral infections. Secreted interferon results in the transcription and production of hundreds of interferon-stimulated genes (ISGs). A genome-wide CRISPR screen using IFN alpha-treated Huh7.5 cells was performed to determine which ISGs were required in order for host cells to suppress yellow fever virus (YFV) infection. Human Huh7.5 cells were transduced with a human CRISPR library in a single replicate at 900X library coverage, treated with IFN alpha, infected with YFV-17D-venus, and GFP-positive cells that escaped IFN-alpha suppression of YFV-17D-venus infection were collected by FACS.