Single-nucleus transcriptomic analysis of the amygdala of mice in acute and chronic neuropathic pain

Single-nucleus RNA sequencing was used to profile transcriptional differences in mice in uninjured or neuropathic chronic pain conditions and in response to multiple sensory stimuli to identify cell subtype-specific changes in the transcriptome due to pain We performed the 10x Genomics 3' gene expression assay (v3.1). Nuclei were isolated from the right amygdala of adult male mice that were either uninjured or 3 weeks following a left side spared nerve injury to induce chronic neuropathic pain. Uninjured mice were split into groups of 3 biological replicates that received either no stimulus or a light touch to the left hindpaw or a noxious hot water droplet to the left hindpaw. Mice with a spared nerve injury were split into two groups of 3 biological replicates that received either no stimulus or a light touch to the left (injured) hindpaw to induce chronic neuropathic pain. Nuclei capture and library preparation was perormed using 10x genomics.