Gene expression levels of evolved thermotolerant yeast and its parent

Samples for RNA-seq were collected from cell cultures in YPD medium under 30 °C or 37 °C at the logarithmic phase. The gene expression levels of thermotlerant yeast and the parent under these two temperatures were analyzed. We found the most upregualted genes of the parent under high temperature versus normal temperature were involved in autophay. the peroxisome pathway was significantly upregulated in G14 under high temperature versus the parent under normal temperature. The antixidant system of yeast was activated under thermal stress. Overall design: the samples were classfied into different comparison groups. The expression level of thermotolerant yeast under 37? versus the parent under 30? were followed.