Distinct roles of LARP1 and 4EBP1/2 in regulating translation and stability of 5'TOP mRNAs [LARP1]

To investigate the changes in gene expression upon loss of LARP1, we performed RNA-seq and differential gene expression analysis on WT and derived LARP1 KO cell lines. The canonical and 5'TOP mRNA cell lines used for single-molecule imaging were treated as biological replicates for the differential gene expression analysis. Overall design: WT cells (canonical and 5'TOP mRNA cell lines) and derived LARP1 KO clones (4 for each cell line, 8 clones total) were analyzed by RNA-seq, using independent replicates (cells harvested on different days).